This manual describes how to use the AccuScript PfuUltra II RT-PCR kit.
The Agilent AccuScript PfuUltra II RT-PCR Kit provides a complete system for reverse transcription-polymerase chain reaction (RT-PCR) applications requiring high fidelity and specificity. The kit includes reagents for synthesizing first-strand cDNA from total or poly(A)+ RNA using AccuScript High Fidelity Reverse Transcriptase, followed by PCR amplification of the cDNA template using PfuUltra II Fusion HS DNA Polymerase.
| Target Length Range | 0.1-9.6 kb |
|---|---|
| Total RNA Input Range | 10-1000 ng |
No, DEPC-treated water should not be used in the cDNA synthesis reaction because DEPC can inhibit PCR.
When using random primers, the first-strand synthesis reaction must be incubated at 25°C for 10 minutes to extend the primers.