Agilent CytoTrap XR Library Construction Kit Instruction Manual

About this manual

The document provides comprehensive instructions for using the CytoTrap XR Library Construction Kit, catalog number 200444. It outlines the materials provided, including specific vectors (pSos, pMyr), host strains, and reagents required for cDNA synthesis and library preparation.

The guide covers step-by-step protocols for preparing yeast host strains, transforming XL10-Gold cells with bait plasmids, and performing cotransformation or mating assays to identify protein-protein interactions. It also includes troubleshooting tips, media preparation instructions, and safety information regarding hazardous materials like methylmercury hydroxide.

What's inside

• Materials Provided
• Storage Conditions
• Preparation of Yeast Host Strain
• cDNA Insert Preparation and Ligation
• Transforming XL10-Gold Cells
• Bait Plasmid Construction
• Cotransformation of Bait and Target Plasmids
• Library Screening
• Verification of Interaction
• Troubleshooting

Specifications

Frequently asked questions

What is the storage temperature for XL10-Gold Kan Ultracompetent Cells?

They should be stored at -80°C.

How many vector-ligated constructs can the reagents generate?

Enough reagents are included to generate five vector-ligated constructs.

Where should the pMyr XR vector be stored upon arrival?

It must be stored at -20°C immediately upon arrival.