Agilent LipoTAXI Mammalian Transfection Kit Instruction Manual

This manual describes the use of the Agilent LipoTAXI Mammalian Transfection Kit.

Agilent LipoTAXI Mammalian Transfection Kit Instruction Manual - cover page
Brand
LipoTAXI
Document type
User Manual
Language
English
Pages
16
File format
PDF
File size
85 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
93096AE0F40A990DA3E7670AD6A95FF6
PDF
Agilent LipoTAXI Mammalian Transfection Kit Instruction Manual PDF

About this manual

This document is an instruction manual for the LipoTAXI Mammalian Transfection Kit (Catalog #204110), manufactured by Agilent Technologies. The kit includes a low-toxicity lipid formulation, a control plasmid (pCMV B-gal), and instructions for use with over 30 tested cell lines.

What's inside

  • Materials Provided
  • Storage Conditions
  • Transfection Protocol
  • Transfecting Adherent Cells
  • Transfecting Suspension Cells
  • B-Galactosidase Activity Test
  • Harvesting Transfected Cells
  • Troubleshooting

Specifications

Kit Capacity30-85 transfections in 60-mm tissue culture dishes
LipoTAXI StorageRoom temperature
Control Plasmid Storage-20°C

Frequently asked questions

What is the maximum number of transfections this kit can perform in a standard dish?

The kit has been optimized for up to 85 transfections when using 60-mm tissue culture dishes.

What is the recommended incubation time after adding the activated solution before replacing the medium?

You should incubate the cells for 24 to 72 hours depending on the cell type and promoter activity.

How often must fresh selection antibiotics be applied during a stable transfection?

Fresh selection antibiotics should be applied every 4 to 7 days, which is approximately two times per week.

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