Complete Control Cell Lines INSTRUCTION MANUAL is a product manual of Agilent.
This document provides instructions for using the Agilent Complete Control Cell Lines, which are part of an inducible mammalian expression system. The kit includes three specific cell lines: ER-CHO (derived from Chinese hamster ovary cells), ER-NIH (mouse fibroblast cells), and ER-293 (human primary embryonal kidney cells). These lines stably express optimal levels of VgEcR and RXR receptors, eliminating the need to produce stable receptor-expressing cell lines for preliminary experiments.
| Catalog Numbers | #222100, #222105, and #222110 |
|---|---|
| Cell Line Source (ER-CHO) | Chinese hamster ovary cells |
| Cell Line Source (ER-NIH) | mouse fibroblast cells |
| Cell Line Source (ER-293) | human primary embryonal kidney cells |
| Receptor Expression Level | Optimal levels of VgEcR and RXR receptors |
| Induction Fold (ER-CHO) | 1,000-fold |
The catalog numbers are #222100 (ER-CHO), #222105 (ER-NIH), and #222110 (ER-293).
Cryovials must be stored immediately in liquid nitrogen upon receipt.
The required G418 concentration for ER-CHO cells is 500 pg/ml.