pMC1neo and pMC1neo Poly A Vectors INSTRUCTION MANUAL
This instruction manual provides guidance for the use of Agilent Technologies pMC1neo and pMClneo Poly A vectors. It details the materials provided with the kit, including specific bacterial strains and vector quantities, and outlines proper storage conditions at -20°C or -80°C.
The document includes step-by-step instructions for preparing host cells from glycerol stocks, creating new -80°C glycerol stocks, and formulating LB broth and agar media. It also provides vector maps and references for further information.
| pMC1neo Quantity | 25 ug |
|---|---|
| pMClneo poly A Quantity | 25 ug |
| Vector Storage Temperature | -20°C |
| Bacterial Glycerol Stock Storage Temperature | -80°C |
The vectors should be stored at -20°C.
The AGI1 strain glycerol stock should be stored at -80°C.