Agilent GeneMorph II Random Mutagenesis Kit Instruction Manual

About this manual

The GeneMorph II Random Mutagenesis Kit is a research tool designed for generating amino acid substitutions in proteins through error-prone PCR. It utilizes Mutazyme II DNA polymerase, a blend of two error-prone enzymes formulated to provide useful mutation rates with minimal mutational bias compared to previous versions.

Users can control the mutation frequency (ranging from 0 to over 16 mutations per kb) by adjusting the initial amount of target DNA and the number of amplification cycles. The kit includes sufficient reagents for 30 reactions, including Mutazyme II polymerase, reaction buffer, dNTP mix, and a gel standard.

What's inside

• MATERIALS PROVIDED
• STORAGE CONDITIONS
• ADDITIONAL MATERIALS REQUIRED
• INTRODUCTION
• Random Mutagenesis with the GeneMorph II Kit
• Mutational Spectrum of the GeneMorph II Kit
• Preprotocol Considerations
• Achieving High Mutation Frequencies
• Troubleshooting
• Quick-Reference Protocol

Specifications

Frequently asked questions

What is the recommended initial target DNA amount for low mutation frequencies?

An initial target amount of 500-1000 ng is recommended to achieve low mutation frequencies of 0-4.5 mutations/kb.

How can high mutation frequencies be achieved?

High mutation frequencies (>9 mutations/kb) are obtained by using 0.1-100 ng of input target DNA.

What additional materials are required to use the kit?

Users need a temperature cycler, PCR tubes, and PCR primers.