TKX1 Competent Cells Instruction Manual is the user manual for the TKX1 Competent Cells product produced by Agilent. It introduces the product's usage methods, precautions, and maintenance methods.
The document is an instruction manual for the Agilent TKX1 Competent Cells (Catalog #200124). It details the materials provided with the kit, such as the cells themselves and a control plasmid, along with necessary storage conditions at -80°C. The text outlines that these cells are a tyrosine kinase derivative of the XL1-Blue MRF' Kan strain.
| Transformation Efficiency | > 5 x 10^7 cfu/ug of pUC18 DNA |
|---|---|
| Storage Temperature | -80°C |
| Recommended Storage Duration | at least 6 months |
Optimal efficiencies are observed when cells are heat-pulsed for 45-50 seconds.
Falcon 2059 polypropylene tubes must be used because other tubes may be degraded by B-mercaptoethanol.
Add 1-5 ul of DNA for a final concentration of 1-100 ng of DNA in the transformation.