Agilent Technologies miRNA Microarray System v2.4 User Manual

About this manual

This document provides recommended procedures for the preparation and labeling of complex biological targets and hybridization, washing, scanning, and feature extraction of Agilent oligonucleotide microarrays. It covers the use of the miRNA Complete Labeling and Hyb Kit with SurePrint Technology microarrays.

The guide details steps including Spike-In solution preparation (optional), sample labeling and purification, drying, blocking agent preparation, hybridization assembly, washing protocols using Gene Expression Wash Buffers, and data extraction using Agilent Feature Extraction Software. It also includes safety notes regarding reagents like DMSO and 2X Hi-RPM Hybridization Buffer.

What's inside

• Procedural Notes
• Safety Notes
• Required Equipment
• Required Reagents
• Spike-In Solution Preparation
• Sample Labeling and Hybridization
• Microarray Wash
• Scanning and Feature Extraction
• Characterization of Total RNA

Specifications

Frequently asked questions

Is this product for diagnostic use?

No, the microarrays are for research use only and not for use in diagnostic procedures.

What safety precautions are required for DMSO?

DMSO may be harmful by inhalation, ingestion or skin absorption and can cause skin irritation.

What is the recommended storage condition for microarray slides after opening?

Store at room temperature in the dark under a vacuum dessicator or N2 purge box.