This document is the user guide for ImageJ 1.45, providing information on the installation, usage, and extension of the ImageJ image processing software.
ImageJ is a public‑domain Java‑based image processing and analysis program that runs on Windows, macOS and Linux. This guide explains how to install, update, and operate ImageJ, as well as how to extend its functionality with macros, scripts, and plugins.
The manual covers getting started, keyboard shortcuts, menu commands, image handling (types, formats, stacks, hyperstacks), processing tools, analysis functions, plugin management, and where to find additional help and resources.
| Supported image bit depths | 8‑bit, 16‑bit, 32‑bit |
|---|---|
| Supported file formats | TIFF, GIF, JPEG, BMP, DICOM, FITS, raw |
| Operating systems | Windows, macOS, Linux |
Run the Help → Update ImageJ… command, which installs the latest ij.jar in the ImageJ folder.
ImageJ can be downloaded from http://imagej.nih.gov/ij/download.html.
ImageJ can read TIFF, GIF, JPEG, BMP, DICOM, FITS and raw files.