This document is a Leica SP5 BASIC user guide, which introduces the startup, use and maintenance of the product.
The guide walks users through the initial startup of the Leica SP5 microscope, including uncovering the instrument, turning on the Hg‑lamp, laser power switch, scan box, and computer, and launching the LAS AF software. It also covers sample preparation, slide loading, and basic illumination and objective handling.
Operational details describe the microscope’s control panel, objective and stage movements, laser and detector configuration in the LAS AF software, and image acquisition settings such as laser power, scan speed, and image format. The final section explains the proper shutdown sequence to protect the lasers and preserve data. This manual is written in Chinese.