This is a user manual for Model 111 Mini IEF Cell
The Model 111 Mini IEF Cell is a compact isoelectric focusing instrument that uses an inverted gel format with two removable graphite electrodes. It runs ultra‑thin 0.4 mm gels without the need for electrode buffers or active cooling, delivering tight protein bands at up to 450 V within 90 minutes.
This manual covers general information, specifications, component description, gel casting procedures, electrophoresis principles, sample preparation, running conditions, cleaning, safety interlocks, warranty details, troubleshooting, and a list of accessories and reagents required for operation.
| Outer chamber material | Fabricated acrylic |
|---|---|
| Electrode material | High purity graphite, 0.95 cm diameter |
| Overall dimensions | 24.6 × 11.4 × 4.8 cm (L × W × H) |
| Casting tray dimensions | 20.3 × 13.9 × 2.3 cm (L × W × H) |
| Gel size | 125 × 65 mm, 0.4 mm thickness |
| Voltage / power limit | 500 VDC / 5 W |
The manual specifies a maximum voltage of 500 VDC and a power limit of 5 W.
Clean the cell components with Bio‑Rad Cleaning Concentrate (catalog 161‑0722) or a mild detergent/ethanol, then rinse thoroughly with distilled water.
Bio‑Rad Laboratories warrants the Model 111 Mini IEF Cell for one year against defects in materials and workmanship, excluding improper operation, unauthorized repairs, and certain consumable parts.