This manual introduces Bio-Rad's Rotofor® system, including the system's components and operating procedures. The system is used for protein electrophoresis separation, which can separate proteins with different pH values.
This manual covers all aspects of the Rotofor system, a laboratory instrument for preparative isoelectric focusing. It includes general information, detailed descriptions of major components, step‑by‑step setup procedures, running conditions, sample preparation, and optimization tips.
Additional sections address analysis of results, a troubleshooting guide, maintenance instructions, and a comprehensive product information list with part numbers and accessories.
| Shipping weight | 9 kg |
|---|---|
| Overall size | 45.7 cm x 16.5 cm x 22.8 cm |
| Cell voltage limit | 3000 VDC |
| Cell power limit | 15 W |
| Maximum coolant flow | 12 L/min |
| Sample volume range | 18–58 ml |
The cell can handle up to 3000 VDC.
A refrigerated circulating water bath should keep the coolant at 4 °C.