This manual provides detailed information on the Helios Gene Gun System, including specifications and performance, components, operating instructions, maintenance and warranty.
The Helios Gene Gun System is a portable particle‑delivery instrument used for research‑grade gene transfer in vitro and in vivo. It accelerates DNA‑coated gold microcarriers with a high‑pressure helium pulse to penetrate cell membranes.
The manual covers general safety information, system components, setup of helium and nitrogen supplies, battery operation, step‑by‑step usage, troubleshooting, specifications, and spare‑part information. Appendices provide detailed procedures for cartridge preparation, cleaning, and quantitative assays.
| Battery type | Standard 9 V alkaline battery |
|---|---|
| Battery life | Approximately 1,000 discharges per battery |
| Helium regulator fitting | CGA 580 female fitting |
It uses a standard 9 V alkaline battery, which should be replaced only after detaching the gun from the helium hose.