The DBI ECL Immunoblot Chemical Luminescence Solution is a laboratory auxiliary reagent for detecting proteins above 30 or 50 pg fixed on solid phase membranes (such as NC, PVDF, etc.). The technology was carefully developed and successfully tested by DBI. It is suitable for immunochemical detection of horseradish peroxidase-labeled proteins. The product is ready to use, has stable performance, is easy to operate, has high sensitivity, clear luminescence and good repeatability.
This manual describes the TOP‑ECL chemiluminescence solution developed by Xinghan Bio for western blot applications. It lists the reagent components (A and B liquids), storage requirements, and the supplies needed by the user such as membranes, conical tubes, and a shaking incubator.
Step‑by‑step experimental procedures are given, including preparation of the working solution, incubation times, and exposure of the membrane to X‑ray film. Important precautions such as protecting the reagents from light, wearing gloves, and adjusting exposure time based on protein abundance are also covered. This manual is written in Chinese.
| Reagent A volume | 25 ml or 50 ml |
|---|---|
| Reagent B volume | 25 ml or 50 ml |
| Recommended storage temperature | 2 °C – 8 °C |
| Total product volume | 50 ml |
| Suggested usage per membrane | 2 ml per 5 × 8 cm² membrane |
Store both Reagent A and Reagent B at 2 °C to 8 °C in the refrigerator and keep them protected from light.
One 50 ml kit can be used for up to 1000 cm² of membrane.
Wear gloves, keep reagents away from light, and use foil or plastic wrap to prevent exposure to light during incubation and exposure steps.