DL2,000TM DNA Marker is a DNA solution containing 1×Loading Buffer, which can be taken directly by 5μl electrophoresis, making it very convenient to use. DL2,000TM DNA Marker is composed of DNA fragments 2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp and 100 bp, a total of 6 bands. When 5μl is taken for electrophoresis, the 750 bp DNA fragment is about 150 ng and shows a bright band. The DNA amount of the remaining bands is about 50 ng.
This manual describes the DL2,000 DNA Marker, which contains six DNA fragments (2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp) for use as a loading marker in agarose gel electrophoresis. It explains how to reconstitute the marker, the recommended loading amounts, and how to achieve clear bands.
The guide also gives storage recommendations (short‑term at 4 °C, long‑term at –20 °C) and practical tips for agarose concentration and well width to obtain optimal separation of the marker bands. This manual is written in Chinese.
| DNA fragment sizes | 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp |
|---|---|
| Typical loading amount per lane | ≈150 ng for 750 bp band, ≈50 ng for other bands |
| Reconstitution concentration | ≈400 ng per 5 µL |
| Recommended agarose gel concentration | 1 %–1.5 % (optimal for fragment range) |
The marker contains six fragments of 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp.
Store short‑term at 4 °C and for long‑term preservation keep it at –20 °C.
A gel concentration of about 1 % to 1.5 % is recommended; higher concentrations improve resolution of short fragments, while lower concentrations aid long fragments.
Use wells narrower than 6 mm; if wells are wider, increase the loading volume accordingly.