Nicomp 380 user manual, introduces the registration information, technical support, safety considerations, CE marking, DLS theory, dynamic light scattering theory, the simplest approach to determine particle size distribution: Gaussian analysis, uniform particle size-simple analysis, wide uniform distribution, multi-peak distribution and other contents.
The Nicomp 380 is a dynamic light scattering (DLS) system designed for particle sizing and molecular weight estimation. This manual provides detailed instructions for registration, safety considerations, hardware setup, software installation, and data analysis using various Gaussian weighting methods.
Key sections include theory of DLS, step‑by‑step software menus, sample preparation guidelines, maintenance procedures, and appendices with solvent tables and molecular‑weight calculation formulas.
| APD overload protection | Enabled |
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The instrument calculates molecular weight from the measured diffusion coefficient using an empirical formula (MW = (α/D)¹/β) where α and β are obtained from solvent‑specific constants or calibration data.
Appendix D provides a table of non‑aqueous solvents with temperature, viscosity, and refractive index values, such as m‑Nitrotoluene (20 °C, 2.33 cPoise, 1.545 index).