20 bp DNA Ladder Marker is a DNA solution that contains 1× Loading Buffer. It can be directly used for electrophoresis after taking 5μl. 20 bp DNA Ladder Marker consists of DNA fragments with lengths of 20 bp, 40 bp, 60 bp, 80 bp, 100 bp, 120 bp, 140 bp, 160 bp, 180 bp, 200 bp, 300 bp, 400 bp, and 500 bp. It has 13 bands, among which 100 bp, 200 bp, and 500 bp are marker bands that show bright bands. This marker is mainly used for electrophoresis of small DNA fragments such as RNA interference, and it is recommended to use post-staining during electrophoresis.
The D521A 20 bp DNA Ladder Marker from Takara is a molecular weight marker used for agarose and PAGE electrophoresis. It contains DNA fragments ranging from 20 bp to 500 bp, allowing size estimation of nucleic acid samples.
The manual describes the recommended loading buffer (500 ng in 5 µl), storage conditions (‑20 °C), and gel preparation guidelines (e.g., 4 % agarose). It also lists the fragment sizes included in the ladder and provides brief handling instructions.
| Size range | 20 bp – 500 bp |
|---|---|
| Loading amount | 500 ng per 5 µl |
| Storage temperature | -20°C |
| Agarose gel concentration | 4% |
The ladder includes fragments from 20 bp up to 500 bp.
Load 500 ng of DNA in a 5 µl volume.
Store the marker at –20 °C.