PstI is a common endonuclease. This product is imported and packed. The basic information is as follows: the recognition sequence is CTGCA^G G^ACGTC. The compatible buffers include Buffer O, Buffer Y, etc. The digestion temperature is 37 °C. The inactivation conditions are 80 °C 20 minutes. The activity unit is defined as the amount of enzyme that completely breaks down 1 μg of λ DNA in a 50-microliter reaction system at 37 °C for 1 hour, which is 1 U.
The document describes the PstI endonuclease (product code D6363) supplied as 1000 U per vial. It defines the activity unit (1 U) as complete digestion of 1 µg DNA in 1 hour at 37 °C and gives basic information on enzyme stability and handling.
Recommended reaction conditions include a 37 °C incubation for 1 hour, with detailed recipes for 1X Buffer O and 1X Buffer Y. Storage guidelines advise keeping the enzyme at –20 °C, and safety notes stress the use of disposable gloves and proper disposal of waste. This manual is written in Japanese.
| Enzyme activity per vial | 1000 U |
|---|---|
| 1X Buffer Y composition | 33 mM Tris‑acetate (pH 7.9 at 37 °C), 10 mM magnesium acetate, 66 mM potassium acetate, 0.1 mg/ml BSA |
| Storage temperature | −20 °C |
Store the enzyme at –20 °C.
Each vial contains 1000 U of PstI enzyme.