XhoI is an endonuclease product with a specific recognition sequence and cutting efficiency. The product is compatible with different buffers and has good cutting and ligation efficiency. Storage and usage require attention to relevant conditions and safety precautions.
This manual describes the XhoI restriction endonuclease (product code D6721) and its recommended usage. It includes the enzyme's recognition sequence, recommended reaction conditions, and detailed buffer formulations (Buffer R and Buffer Y) for optimal digestion.
Safety notes, storage recommendations (‑20 °C), and unit activity definition are also provided, along with packaging details and guidelines for multi‑step digestions. This manual is written in Japanese.
| Recognition sequence | CTCGAG |
|---|---|
| Buffer Y composition | 33 mM Tris‑acetate (pH 7.9), 10 mM magnesium acetate, 66 mM potassium acetate, 0.1 mg/ml BSA |
XhoI recognizes the sequence CTCGAG.