PrimeSTAR® HS DNA Polymerase with GC Buffer is used for high-fidelity amplification of complex secondary structure DNA templates (GC rich, repetitive sequences, etc.) and has a strong 3′→5′ Exonuclease activity and high amplification efficiency superior to Taq DNA Polymerase. This product can play a strong role in the PCR reaction requiring high fidelity, such as cDNA cloning, and can efficiently amplify high GC templates. PCR products amplified using this product are almost all smooth-ended and can be directly cloned into vectors with smooth ends after phosphorylation.
This manual describes the PrimeSTAR HS DNA Polymerase with GC Buffer, a high‑fidelity enzyme designed for amplifying GC‑rich templates. It lists the kit contents, enzyme activity, and recommended buffer composition.
Detailed PCR reaction mixtures, cycling parameters for 2‑step, 3‑step, and 4‑step protocols, and storage guidelines are provided, along with example amplifications of APO E and Z7 HB8 genes. Precautions for reagent preparation and handling are also included. This manual is written in Chinese.
| Enzyme activity per reaction | 2.5 U |
|---|---|
| Buffer concentration | 2x PrimeSTAR GC Buffer |
| dNTP concentration | 2.5 mM each |
| Mg2+ concentration | 2 mM |
Each reaction uses 2.5 U of PrimeSTAR HS DNA Polymerase.
Add Mg2+ to a final concentration of 2 mM.
Use 2× PrimeSTAR GC Buffer in the reaction mixture.