This manual introduces the basic use of Primer PREMIER Version 5.0 for Windows and Power Macintosh software.
Primer Premier is a software package that helps researchers design optimal PCR primers. It offers functions such as advanced primer search, a primer database, nested primer design, sequence editing, alignment, restriction‑enzyme analysis and motif analysis.
The manual describes the GeneTank sequence‑editing window, the Primer design interface, Enzyme and Motif analysis windows, parameter settings, system requirements and how to obtain technical support. It also includes appendices with formulae, degenerate base tables, amino‑acid codes, software limits and references. This manual is written in Chinese.
| Maximum primer length | 70 nt |
|---|---|
| Maximum sequence length | 50 kb |
| Maximum restriction‑enzyme site length | 19 |
| Maximum primer pair search | 1000 |
| Supported Windows OS | Windows 98/NT 4/2000 |
| Supported Macintosh OS | Power Macintosh 7.0 or later |