This document describes the features and functions of Agilent MassHunter Workstation software. The software enables fast and easy transformation of mass spectrometry data into answers, and provides various functionalities including data acquisition, instrument control, data mining, and processing. It is compatible with Agilent LC/MS, GC/MS, and ICP-MS instruments, and offers a unified user interface and comprehensive software modules for quantitative analysis, compound confirmation, target compound screening, degradant and unknown identification, biomolecule characterization, as well as protein and metabolite identification workflows.