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This manual mainly introduces the safety, introduction, test preparation, basic operation, technical specifications, system function list, interface definition, maintenance and appendices of the test instrument.
N8020A is a high-performance gigabit Ethernet switch card customized for the N8000 system, which serves as the data bridge between the functional boards and PC in the N8000 device.
The manual of Vxiaobao property APP is about how to use Vxiaobao property APP. It mainly introduces the download, registration, function introduction and other content of APP, which can be used as a reference for users.
The 788T High Resolution Digital Audio Recorder is a professional audio recorder from Sound Devices. The device features a compact design for easy portability, and offers recording quality up to 192kHz/24-bit. The 788T is equipped with two full-size XLR inputs, two 1/4-inch TRS inputs, and a 1/8-inch auxiliary input. Additionally, the device supports SD cards and USB storage.
A method for preparing NY-ESO-1-specific TCR. A fruit fly antigen presenting cell is loaded with NY-ESO-1 antigen peptide and activated CD8+T cells to produce CTL cells. The cytotoxic CTL cells are then stained with HLA-A*02:01 NY-ESO-1 Tetramer-SLLMWITQC-APC (MBL, TS-M011-2) and CD8-FITC fluorescent double staining, and the flow cytometry assay is used to screen and collect dual positive CTL cells. The selected CTL cells are then subjected to single-cell TCR gene sequencing to obtain TCRα and β variable region gene sequences. The TCR genes are then optimized and designed, and the constant region of the TCR genes is converted to a murine source and supplemented with signal peptides to obtain the full gene sequence of TCRα and β chains. TCR α and β chains are genetically synthesized and connected with the IRES sequence to ensure that TCRα and TCR β chains are co-expressed in a plasmid to obtain TCR genes. The TCR genes are then expressed in eukaryotes.