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- Last added
- 2026-10-17
Featured HanBio manuals
HanBio Lentivector Construction and Packaging Operation Manual
This manual provides a comprehensive guide for laboratory personnel on constructing lentivectors and packaging them using a three-plasmid system. It covers the preparation of shuttle plasmids and packaging vectors, followed by transfection into 293T packaging cells. The process includes detailed steps for cell culture, transfection using LipoFiter reagents, and the collection of viral supernatants. This manual is written in Chinese.
Covers: Experimental Process · Experimental Materials · 293T Cell Culture · Virus Packaging and Concentration · Infection of Target Cells · Polybrene Usage
Latest HanBio manuals
HanBio Lentivector Construction and Packaging Operation Manual
User Manual 26 pages · 470 KB · ZHThis manual details the procedures for constructing lentivectors, packaging them in 293T cells, and infecting target cells, including virus concentration…
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in HanBio manuals
What is the recommended Polybrene working concentration?
The most common working concentration for Polybrene is 6～8μg/ml.
From: HanBio Lentivector Construction and Packaging Operation Manual
How should the virus supernatant be collected after transfection?
Collect the viral supernatant at 48 and 72 hours post-transfection.
From: HanBio Lentivector Construction and Packaging Operation Manual