G4240-62001 HPLC-Chip is the standard chip for one-dimensional separations. It is comprised of a 40 nL enrichment column and a 75 μm x 43mm separation column which is packed with Zorbax 300SB-C18 5 μm material. The HPLC-Chip is made from biocompatible polyimide and the functionality of this chip is equivalent to conventional nanospray LC/MS. ZORBAX 300SB-C18 is a micro particulate column packing designed specifically for reversed-phase HPLC of peptides and proteins. The StableBond packing is made by chemically bonding a sterically protected octadecyl stationary phase to a specially prepared, ultra-high- purity, ZORBAX, porous-silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected diisobutyl n-octadecyl stationary phase is chemically stable and gives longer column life. As a result, ZORBAX 300SB-C18 is a stable, reversed-phase packing at low pH that can be used for peptide maps, and separation of synthetic and natural peptides and proteins. It is particularly well suited for use with aggressive mobile phases (e.g., pH < 2, high ionic strength, ion-pair additives, TFA, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This characteristic is particularly important for use in methods that need long-term stability and reproducibility. ZORBAX 300SB-C18 is well suited to applications that utilize weak acidic ion-pair additives as the mobile phase, such as those used for peptide analysis with TFA.
