Update: 30 September, 2023
Agilent 6410 Triple Quadrupole Mass Spectrometer (QQQ) is used to analyze several water-soluble B-vitamin compounds in breakfast cereal, including thiamine, riboflavin, pyridoxine, vitamin B12, pantothenic acid, biotin, and niacinamide, which are present at relatively high levels. A simple gradient elution is carried out on a Rapid Resolution High Throughput SB-Aq column (particle size 1.8 µm). All compounds elute in less than 7.5 minutes, and with the exception of pyridoxine, good linearity over more than three orders of magnitude, from 0.5 to 500 ppb, is demonstrated with good peak area reproducibility at the 0.5 ppb level, which is the lowest level of quantitation considered.
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Publication date: 12 June, 2012
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