Update: 28 September, 2023
A method for simultaneous determination of malachite green and crystal violet, and their metabolites leuco-malachite green and leuco-crystal violet in fish, was developed and validated. The analytes were extracted by solid phase extraction and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry operating in positive ion multiple reaction monitoring mode. The method provided a 0.5 ng/g limit of quantification for all these compounds in fish. The dynamic calibration ranges for these compounds were obtained from 0.5 to 100 ng/g. The overall recoveries ranged from 96 to 109% with RSD values between 1.7 and 4.5%.
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MD5 Checksum: 112E1791D395490038D9B02F8C56A776
Publication date: 11 June, 2012
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PDF Link: Agilent LC/MS of Malachite Green Crystal Violet in Fish with Agilent Bond Elut PCX Poroshell 120 PDF