Update: 28 September, 2023
The Varian Evaporative Light Scattering Detector is a powerful tool for detecting any sample that is less volatile than the mobile phase in HPLC applications, irrespective of the optical properties of the compounds of interest. The ELSD offers distinct advantages over more conventional UV or DAD detection, particularly for gradient separation. In the past, one disadvantage for some ELSD designs has been that high operating temperatures required to fully evaporate the eluent can cause loss of semi-volatile, small molecules, e.g. drug candidates. The Varian Evaporative Light Scattering Detector features design improvements to overcome these drawbacks. For the detection of semi-volatile components in a sample, the Varian ELSD can be operated with the heaters off (ambient temperature operation) or with the nebulizer and evaporator controlled at low temperatures. Figure 1 shows a separation of a test mixture of four compounds by reversed phase HPLC, which is typically used to assess HPLC system performance for pharmaceutical analysis.
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Publication date: 05 July, 2012
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PDF Link: Agilent Semi-Volatile Pharmaceutical Compound Analysis using Low Temp ELSD Manual PDF