Update: 30 September, 2023
The Varian 385-LC is an advanced evaporative light scattering detector from Polymer Laboratories, now a part of Varian, Inc. The Varian 385-LC delivers sub-ambient evaporation down to 10 °C, providing maximum sensitivity for compounds with significant volatility below ambient temperature. The instrument benefits from fast data output rates and extremely low dispersion for fast LC, and delivers a universal response down to the low-nanogram range for truly representative analysis. Reproducibility is less than 2% for improved consistency of results. The Varian 385-LC offers real time gas management that eliminates solvent effects to give a constant response across a gradient. Control and digital data collection come as standard for multi-vendor platforms and so there is no need for an analog to digital converter. On-the-fly adjustment of light source intensity saves time during a run, too - all this in the smallest footprint around. Being complementary to LC/MS, and offering unrivalled flexibility and sensitivity, the Varian 385-LC is the ELSD of choice for the most demanding applications. The success of ELS detection relies on evaporating the eluent without destroying the analyte particle. Where the analyte is non-volatile, evaporator temperatures of 50-100 °C can be used without compounds degrading, thus giving maximum sensitivity. However, when compounds have high vapor pressures and the eluent has a high boiling point (eg water), since the evaporation rate of the eluent is much lower than that of the analyte, the light scattering signal will be reduced. The Varian 385-LC uses a unique condenser design to provide high sensitivity in this case.
File format: PDF
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MD5 Checksum: 1C71DFFB8644E9D3FE43AF85AABB660B
Publication date: 05 July, 2012
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PDF Link: Agilent Overcoming Compound Volatility with Sub-ambient ELSD Manual PDF