Update: 28 September, 2023
Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 is especially useful for the separation of acidic, basic, and other highly polar compounds by reverse-phase liquid chromatography. Eclipse Plus C18 packing is made by first chemically bonding a dense monolayer of dimethyl-n-octadecylsilane stationary phase to a specially prepared, an improved ultra-high purity (>99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded-phase packing is then doubly endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C18 columns can be used for acidic and neutral samples, but are especially suited for separating basic compounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2–9, accommodating most popular mobile phases. The uniform, spherical, Eclipse Plus C18 particles are based on an improved ZORBAX Rx-SIL support that has a nominal surface area of 160 m²/g and a controlled pore size of 95Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure slurry-loading technique to give maximum column efficiency.
File format: PDF
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MD5 Checksum: 0F114314C2F83F5709551AD3A1CB427C
Publication date: 04 July, 2012
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PDF Link: Agilent ZORBAX Eclipse Plus C18 Data Sheet PDF