Update: 30 September, 2023
Agilent Poroshell 300SB-C3 is a unique microparticulate superficially-porous C3 column packing from Agilent. Columns of this material are especially useful for carrying out ultra-fast gradient separations of macromolecules because of the favorable kinetic properties of the special silica support. Very rapid gradient separation of proteins, poly-peptides and related bio-macromolecules is attained by operating these columns at high flow rates (mobile phase velocities); rapid mass transfer maintains good column efficiency for excellent separations. This StableBond (SB) packing is made by covalently bonding a patented sterically-protected C3 stationary phase to the specially prepared, high-purity Poroshell 300 microparticles. The special silica support is designed to reduce or eliminate strong adsorption of basic compounds, and is especially friendly towards biomolecules such as proteins. The densely covered, sterically-protected triisopropylsilane stationary phase allows these particles to be routinely used at low pH (for example, pH 1) and temperatures to at least 70 °C with excellent stability. Therefore, a Poroshell 300SB-C3 is particularly well suited for use with aggressive mobile phases that are widely used for the reversed-phase separation of proteins and other such biomacromolecules (for example, pH < 2, high ionic strength, ion-pair additives, and so forth). This packing also is useful for separating compounds such as proteins.
File format: PDF
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MD5 Checksum: 97EBFD55171D5B07FF353C8C2A9E8B0F
Publication date: 04 July, 2012
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PDF Link: Agilent Poroshell 300SB-C3 Data sheet PDF