Update: 29 September, 2023
This document describes how to minimize photobleaching of Blue Fluorescent Protein (BFP) using the Agilent Cary Eclipse fluorescence spectrophotometer. Photobleaching refers to the loss of fluorescence emission from samples due to prolonged exposure to exciting radiation. To maintain optimal fluorescence emission, it is important to minimize photobleaching. Strategies to achieve this usually involve limiting the time of exposure to, or the intensity of, the exciting light. However, these strategies may compromise the signal to noise ratio (S/N), affecting the quality of results or limiting the types of analyses that can be performed. Additionally, extended exposure to excitation light can result in photobleaching, making it challenging to perform kinetics-based assays over a long period of time.
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Publication date: 04 July, 2012
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