Update: 28 September, 2023
Absolute quantitation with the Agilent 2100 Bioanalyzer and the Protein 200 LabChip® kit. The Protein 200 LabChip® kit allows sizing and quantitation of proteins ranging in size from 14 to 200 kD. Relative quantitation is performed in comparison to the upper marker, used as internal standard in each sample. However, the accuracy of the relative quantitation is affected by the staining efficiency of the protein dye. Absolute quantitation is enabled with the Agilent 2100 bioanalyzer software (revision A.02.01) using the Protein 200 assay in combination with protein standards of known concentrations. Absolute quantitation improves quantitation accuracy by eliminating the differences in staining efficiency among proteins. This Application Note describes how to perform absolute quantitation with the Protein 200 assay and demonstrates the obtained improvement in accuracy.
File format: PDF
Size: -
MD5 Checksum: 03EB97ED47B397767A0C4E074C18009F
Publication date: 03 July, 2012
Downloads: -
PDF Link: Agilent Absolute quantitation with the Agilent 2100 Bioanalyzer the Protein 200 LabChip kit PDF