Agilent Second derivative spectral identification of tryptophan tyrosine in peptides

Update: 30 September, 2023

Peak 2 was correctly identified to contain only tyrosine, peak 3 contained neither tyrosine nor tryptophan and peaks 4 and 6 contained only tryptophan (figure 1). Second derivative spectra can be used to emphasize small spectral differences in similar molecules. This is especially useful for identifying tryptophan and tyrosine residues in peptides (figure 2).

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Publication date: 03 July, 2012

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