Update: 29 September, 2023
The document discusses the effect of bonded-phase on the resolution and retention of polypeptides. It highlights that differences in bonded-phase selectivity can significantly improve the resolution in reversed-phase analysis of peptides and proteins. The separation between peaks 2 and 3, peaks 5 and 6, and peaks 8 and 9 illustrate these selectivity changes. Additionally, it mentions that temperature can also be used to achieve desired separations. The steric protection of ZORBAX SB packings makes them well suited for chromatography at very low pH.
File format: PDF
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MD5 Checksum: 7E4ECDB549E06CCE5F1ADEE789F9541A
Publication date: 03 July, 2012
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PDF Link: Agilent Effect of Bonded-Phase on Resolution Retention of Polypeptides PDF