Update: 29 September, 2023
The document describes a method for high-performance liquid chromatography (HPLC) analysis using ion-pair reversed-phase separations combined with electrochemical detection to measure the levels of catecholamines and indoleamines in brain tissue. The method is rapid, selective, and efficient, allowing for analysis of samples in less than 10 minutes per sample. The use of a highly stable column-packing material (Agilent ZORBAX SB-C8, 3.5 µm) ensures unmatched column lifetime, enabling analysis of thousands of samples per column. Samples are directly injected as perchloric acid (0.1N) extracts, eliminating the need for sample workup. This method is suitable for the determination of various biogenic amines neurotransmitters and their metabolites.
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MD5 Checksum: 753A7E0F3146D19E94B6F19F17BF1F2A
Publication date: 03 July, 2012
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PDF Link: Agilent Biogenic Amines Catecholamines PDF