Agilent Comparison of Denaturing non-Denaturing Mobile Phases in SEC: ZORBAX GF-450 /- GuCl log(m.w.)

Update: 30 September, 2023

Size-exclusion chromatography is a powerful technique for the size-dependent separation of biomolecules. While standard, non-denaturing mobile phases are often desirable, denaturing mobile phases containing guanidine hydrochloride (or SDS), may be used to insure un-aggregated, monomers for proper size determination. As shown below, denaturation shifts molecules toward larger apparent size and smaller retention volume.

---

File format: PDF

Size: -

MD5 Checksum: E5BBB0EF78CE6338E29C56F893751562

Publication date: 03 July, 2012

Downloads: -

PDF Link: Agilent Comparison of Denaturing non-Denaturing Mobile Phases in SEC: ZORBAX GF-450 /- GuCl log(m.w.) PDF