Agilent Comparison of Denaturing non-Denaturing Mobile Phases in SEC: ZORBAX GF-250 /- SDS

Update: 30 September, 2023

Size-exclusion chromatography is a powerful technique for the size-dependent separation of biomolecules. While standard, non-denaturing mobile phases are often desirable, denaturing mobile phases containing guanidine hydrochloride (or SDS), may be used to insure un-aggregated, monomers for proper size determination. As shown below, denaturation shifts molecules toward larger apparent size and smaller retention volume.

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Publication date: 03 July, 2012

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PDF Link: Agilent Comparison of Denaturing non-Denaturing Mobile Phases in SEC: ZORBAX GF-250 /- SDS PDF