Agilent USP Method for HPLC - Analysis of Methotrexate

Update: 30 September, 2023

This application note describes a HPLC method for the analysis of methotrexate. The method uses a 10/90 acetonitrile/pH 6.0 phosphate/citrate buffer mobile phase and a ZORBAX Eclipse XDB-C18 column, column dimensions 3.9 x 300 mm, particle size 10 µm.


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Publication date: 03 July, 2012

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PDF Link: Agilent USP Method for HPLC - Analysis of Methotrexate PDF

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