Update: 28 September, 2023
This article describes the use of high-performance liquid chromatography (HPLC) for the analysis of fat-soluble vitamins. HPLC can separate and detect these compounds at room temperature while blocking oxygen and light. UV-visible diode-array detection provides both qualitative and quantitative data. Electrochemical detection is also a highly sensitive and selective HPLC method. Different food matrices require different extraction procedures, including alkaline hydrolysis, enzymatic hydrolysis, alcoholysis, direct solvent extraction, and supercritical fluid extraction.
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MD5 Checksum: 57F0784F91F7BE381F4B9CFC201AFE18
Publication date: 03 July, 2012
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PDF Link: Agilent Analysis of Fat Soluble Vitamins using HPLC PDF