Agilent Mass-based fraction collection of synthetic peptides using the Agilent 1100 Series purification system

Update: 30 September, 2023

This Application Note demonstrates mass-based purification of a series of synthetic peptides with the Agilent 1100 Series purification system. The modular set-up of the system, a reliable fully-automated delay volume calibration and a comprehensive software package provide a versatile platform for purification tasks up to 100 mL/min. Crude peptides were successfully purified by reverse phase HPLC from less than 1 kDa to more than 10 kDa, covering a pI range from 4 to 13.


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Publication date: 02 July, 2012

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