Update: 30 September, 2023
This application note describes the use of the Agilent RNA 6000 Pico assay for the assessment of genomic DNA contamination in total RNA isolated from embryonic kidney cells using laser capture microdissection (LCM). Integrity and relative concentration of total RNA were analyzed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico assay. An unexpected peak between the 18S and 28S ribosomal RNA peaks was observed and unambiguously proven, by DNase digestion and genomic DNA spike-in experiments, to be caused by genomic DNA contamination. A simple on-column DNase digestion method is suggested to eliminate contaminating genomic DNA from total RNA samples obtained by LCM, fluorescent-activated cell sorting (FACS), or manual dissection.
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MD5 Checksum: 92E5B0BB58D5F89F6304FE597A061735
Publication date: 02 July, 2012
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