Update: 30 September, 2023
This Application Note describes an improved method for protein identification with two-dimensional nano LC/MS, which utilizes the elution of digest peptides from a strong cation exchange column by an injected salt step gradient of increasing salt concentration with a subsequent reversed phase separation. However, in this approach the ion exchange chromatography does not produce its full performance. To improve the SCX chromatography performance a new method was developed, which works with a semi-continuous pumped salt gradient. This Application Note describes the improved method for two-dimensional nano LC/MS. To show the full performance of the method a complex tryptic digest of the yeast proteome was analyzed. The results obtained with the developed method are compared to the formerly used injection method as well as to an off-line 2D LC method.
File format: PDF
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MD5 Checksum: 2603ABE8E46C77EB0CAD857741662EC8
Publication date: 02 July, 2012
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PDF Link: Agilent Improved 2D nano LC/MS for proteomics applications - A comparison on yeast PDF