Agilent Technologies Recycle chromatography with the Agilent 1100 Series purification system

Update: 01 October, 2023

This Application Note details how the separation of enantiomers, stereoisomers or diastereomers can be improved by recycle chromatography. The configuration of the system using two identical columns and a 2-position/6-port valve is shown, and the scope and limitations of the system are explained. An application example, which demonstrates the improvement in purity of the collected fractions when using recycle chromatography, is described.


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Publication date: 02 July, 2012

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