Update: 28 September, 2023
The document introduces a fast, convenient, and cost-effective preparative method development that can be achieved by scaling up from analytical HPLC separation conditions. The peak shape and separation of three antibiotics - dicloxacillan, cloxacillan, and oxacillan - are evaluated on four different ZORBAX bonded-phase selectivities, and the best separation method is scaled up in a linear manner to a 21.2 × 150 mm, 5 µm, ZORBAX PrepHT Eclipse XDB-C18 preparative column. When following the suggested easy scale-up procedure, the preparative separation results provide high yields with excellent throughput and purity.
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MD5 Checksum: 0EEB0B81BFDD7996EA9CB9B123665AAE
Publication date: 02 July, 2012
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PDF Link: Agilent Technologies Preparative HPLC Scale-Up of Antibiotics PDF