Agilent Technologies Use of Temperature to Increase Resolution in the Ultrafast HPLC Separation of Proteins with ZORBAX Poroshell 300SB-C8 HPLC Columns.

Update: 30 September, 2023

This paper introduces a method for separating proteins using ZORBAX Poroshell 300SB columns under low pH conditions at high temperatures. The method uses a linear gradient elution method with a flow rate of 1.0 mL/min and a column temperature of 40 °C to 75 °C. The results show that the resolution of the peaks is significantly improved as the column temperature is increased.

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Publication date: 02 July, 2012

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PDF Link: Agilent Technologies Use of Temperature to Increase Resolution in the Ultrafast HPLC Separation of Proteins with ZORBAX Poroshell 300SB-C8 HPLC Columns. PDF