Agilent Technologies Rapid HPLC Analysis of Monoclonal Antibody IgG1 Light Chains Using ZORBAX Poroshell 300SB-C8.
Update: 28 September, 2023
Antibodies are a class of proteins that play a key role in immunological interactions. They bind to antigens (proteins, glycoproteins, DNA, etc.) with high specificity. Antibodies have high value in diagnostics, research, and therapeutics. IgG is a class of antibodies consisting of two heavy chains (50 kDa each) and two light chains (25 kDa each) attached through disulfide bridges. While the carboxy-terminal domains are conserved, the amino-terminal domains have variable amino acid sequences, resulting in the molecule's specificity and diversity. Most antibodies are glycosylated, further increasing molecular diversity. Treatment of intact antibodies with chemicals and enzymes allows specific cleavage of the heavy and light chains, removal of carbohydrate moieties, and cleavage of the polypeptide chains, enabling the study of a particular antibody's structure. Rapid HPLC method development and analysis using ZORBAX Poroshell technology are valuable tools for investigating antibody structure. Rapid HPLC analysis of monoclonal antibody IgG1 light chains using ZORBAX Poroshell 300SB-C8.
Brand: Agilent
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MD5 Checksum: 0BE58DB57F65C17B2AF603A14C18A171
Publication date: 02 July, 2012
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