Agilent Total RNA Isolation from Yeast Cells

Update: 30 September, 2023

This method uses the Agilent Total RNA Isolation Mini Kit to isolate high-purity RNA from yeast cells. The method has the advantage of removing cellular contaminants such as proteins, carbohydrates, and genomic DNA, resulting in a purer RNA product. The method uses a carefully prepared yeast spheroplast followed by complete homogenization of the sample to remove high levels of cell wall components and other proteins, thus achieving high efficiency in the separation of yeast RNA. Compared with other commercial methods, the Agilent method yields comparable yeast total RNA yield and contains significantly less genomic DNA than RNA isolated with a competitor's silica-based kit. Agilent 2100 Bioanalyzer analysis showed that the isolated RNA had high quality and integrity.

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Brand: Agilent

File format: PDF

Size: 101 KB

MD5 Checksum: F2E0FA6EFB8231BF2F7DE0BA74BDA16B

Publication date: 02 July, 2012

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PDF Link: Agilent Total RNA Isolation from Yeast Cells PDF