Agilent Mass-based fraction collection at high flow rates for the purification of compounds in the lower gram scale

Update: 01 October, 2023

This Application Note demonstrates the mass-based purification of low gram amounts of compounds using the Agilent 1100 Series purification platform. Starting the experiments on 21.2-m i.d. columns two application examples were scaled up to a 50-mm i.d. column operated at 100 mL/min. Fractions with purities of 85 and 95 % were obtained by mass-based fraction collection even though the column overload did not allow the UV-trace to show separated peaks.

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MD5 Checksum: B110D57A4A5E48FC2AD261A13DCC7FB5

Publication date: 02 July, 2012

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PDF Link: Agilent Mass-based fraction collection at high flow rates for the purification of compounds in the lower gram scale PDF