Update: 29 September, 2023
This document describes Agilent's dual-mode Gene Expression Microarray platform and the method for gene expression analysis using RNA isolated from human whole blood. The platform combines the ease and simplicity of one-color experimental design with the capability of two-color sensitivity for detection of small gene expression changes. Agilent's platform now includes a method for the analysis of differential gene expression from human whole blood, which is compatible with the PAXgene™ Blood RNA System. The PAXgene Blood RNA System consists of a blood collection tube and nucleic acid purification kit for the collection, storage, and transport of blood and stabilization of intracellular RNA for subsequent isolation and purification. Combined with the PAXgene System, Agilent's amplification and labeling technology, in conjunction with the high sensitivity and specificity of 60-mer oligonucleotide microarrays, offer a total solution for gene expression in whole blood without the need for additional steps or kits to remove globin transcripts. Titration experiments using Agilent RNA spike-in control targets demonstrate the sensitivity of detection.
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Publication date: 02 July, 2012
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